Revisiting biomarker discovery by plasma proteomics

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  • Philipp E Geyer
    Department of Proteomics and Signal Transduction Max Planck Institute of Biochemistry Martinsried Germany
  • Lesca M Holdt
    Institute of Laboratory Medicine University Hospital LMU Munich Munich Germany
  • Daniel Teupser
    Institute of Laboratory Medicine University Hospital LMU Munich Munich Germany
  • Matthias Mann
    Department of Proteomics and Signal Transduction Max Planck Institute of Biochemistry Martinsried Germany

書誌事項

公開日
2017-09
権利情報
  • http://creativecommons.org/licenses/by/4.0/
DOI
  • 10.15252/msb.20156297
公開者
Springer Science and Business Media LLC

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説明

<jats:title>Abstract</jats:title> <jats:p> Clinical analysis of blood is the most widespread diagnostic procedure in medicine, and blood biomarkers are used to categorize patients and to support treatment decisions. However, existing biomarkers are far from comprehensive and often lack specificity and new ones are being developed at a very slow rate. As described in this review, mass spectrometry ( <jats:styled-content style="fixed-case">MS</jats:styled-content> )‐based proteomics has become a powerful technology in biological research and it is now poised to allow the characterization of the plasma proteome in great depth. Previous “triangular strategies” aimed at discovering single biomarker candidates in small cohorts, followed by classical immunoassays in much larger validation cohorts. We propose a “rectangular” plasma proteome profiling strategy, in which the proteome patterns of large cohorts are correlated with their phenotypes in health and disease. Translating such concepts into clinical practice will require restructuring several aspects of diagnostic decision‐making, and we discuss some first steps in this direction. </jats:p>

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