Selective Colorimetric Detection of Polynucleotides Based on the Distance-Dependent Optical Properties of Gold Nanoparticles

DOI Web Site 被引用文献114件
  • Robert Elghanian
    Department of Chemistry, Northwestern University, Evanston, IL 60208, USA.
  • James J. Storhoff
    Department of Chemistry, Northwestern University, Evanston, IL 60208, USA.
  • Robert C. Mucic
    Department of Chemistry, Northwestern University, Evanston, IL 60208, USA.
  • Robert L. Letsinger
    Department of Chemistry, Northwestern University, Evanston, IL 60208, USA.
  • Chad A. Mirkin
    Department of Chemistry, Northwestern University, Evanston, IL 60208, USA.

書誌事項

公開日
1997-08-22
DOI
  • 10.1126/science.277.5329.1078
公開者
American Association for the Advancement of Science (AAAS)

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説明

<jats:p>A highly selective, colorimetric polynucleotide detection method based on mercaptoalkyloligonucleotide-modified gold nanoparticle probes is reported. Introduction of a single-stranded target oligonucleotide (30 bases) into a solution containing the appropriate probes resulted in the formation of a polymeric network of nanoparticles with a concomitant red-to-pinkish/purple color change. Hybridization was facilitated by freezing and thawing of the solutions, and the denaturation of these hybrid materials showed transition temperatures over a narrow range that allowed differentiation of a variety of imperfect targets. Transfer of the hybridization mixture to a reverse-phase silica plate resulted in a blue color upon drying that could be detected visually. The unoptimized system can detect about 10 femtomoles of an oligonucleotide.</jats:p>

収録刊行物

  • Science

    Science 277 (5329), 1078-1081, 1997-08-22

    American Association for the Advancement of Science (AAAS)

被引用文献 (114)*注記

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