<i>Porphyromonas gingivalis</i>Infection during Pregnancy Increases Maternal Tumor Necrosis Factor Alpha, Suppresses Maternal Interleukin-10, and Enhances Fetal Growth Restriction and Resorption in Mice

DOI Web Site 被引用文献9件

書誌事項

公開日
2003-09
権利情報
  • https://journals.asm.org/non-commercial-tdm-license
DOI
  • 10.1128/iai.71.9.5156-5162.2003
公開者
American Society for Microbiology

この論文をさがす

説明

<jats:title>ABSTRACT</jats:title><jats:p>Epidemiological studies have shown a potential association between maternal periodontitis and pregnancy complications. We used a pregnant murine model to study the effect of infection with the periodontal pathogen<jats:italic>Porphyromonas gingivalis</jats:italic>on pregnancy outcomes. Female BALB/c mice were inoculated with heat-killed<jats:italic>P. gingivalis</jats:italic>(10<jats:sup>9</jats:sup>CFU) in a subcutaneous chamber and mated 2 weeks later. At gestation day (GD) 7.5, mice were challenged with live<jats:italic>P. gingivalis</jats:italic>(10<jats:sup>7</jats:sup>CFU) (<jats:italic>n</jats:italic>= 20) or broth (control;<jats:italic>n</jats:italic>= 8) and sacrificed at GD 16.5. Fetal growth restriction (FGR, <0.46 g) was defined as fetuses with weights 2 standard deviations (SD) smaller than controls (0.56 ± 0.05 g [mean ± SD]). Among the 20 challenged mice, 8 had both normal-weight (0.51 ± 0.11 g) and FGR (0.34 ± 0.1 g) fetuses within the same litter. All other challenged dams had normal-weight fetuses (0.57 ± 0.04 g). Maternal liver, uterus, and spleen samples were examined for<jats:italic>P. gingivalis</jats:italic>DNA using a PCR technique. Of the eight challenged mice with FGR fetuses, three had PCR signals for<jats:italic>P. gingivalis</jats:italic>in liver and uterus, but not in the spleen. Liver, uterus, and spleen were negative for<jats:italic>P. gingivalis</jats:italic>DNA among all other challenged and control mice. In serum of dams with FGR fetuses, tumor necrosis factor alpha levels were elevated significantly, while interluekin-10 levels were significantly reduced compared to levels in dams with normal fetuses.<jats:italic>P. gingivalis</jats:italic>-specific serum immunoglobulin G levels were significantly elevated in dams with FGR fetuses compared to dams without any FGR fetuses. These data demonstrate that<jats:italic>P. gingivalis</jats:italic>-induced murine FGR is associated with systemic dissemination of the organism and activated maternal immune and inflammatory responses.</jats:p>

収録刊行物

被引用文献 (9)*注記

もっと見る

詳細情報 詳細情報について

問題の指摘

ページトップへ