Formation of 8‐nitroguanine in DNA treated with peroxynitrite in vitro and its rapid removal from DNA by depurination

Vladimir Yermilov, Julieta Rubio, Hiroshi Ohshima

doi:10.1016/0014-5793(95)01281-6

Formation of 8‐nitroguanine in DNA treated with peroxynitrite in vitro and its rapid removal from DNA by depurination

DOI Web Site Web Site Web Site 被引用文献39件

書誌事項

公開日: 1995-12-04

権利情報

http://onlinelibrary.wiley.com/termsAndConditions#vor

DOI

10.1016/0014-5793(95)01281-6

公開者: Wiley

この論文をさがす

CiNii Books

説明

<jats:p>Peroxynitrite is a strong oxidant formed by reaction of nitric oxide with superoxide in inflamed tissues. We have demonstrated that 8‐nitroguanine is formed dose‐dependently in calf thymus DNA incubated with low concentrations of peroxynitrite in vitro. 8‐Nitroguanine in acid‐hydrolyzed DNA was chemically reduced into 8‐aminoguanine, which was analyzed using high performance liquid chromatography with electrochemical detection. Only peroxynitrite, but not nitrite, tetranitromethane nor NO‐releasing compounds, formed 8‐nitroguanine. Antioxidants and desferrioxamine inhibited the reaction. 8‐Nitroguanine was depurinated from DNA incubated at pH 7.4, 37°C (<jats:inline-graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="graphic/feb20014579395012816-math-si1.gif" xlink:title="urn:x-wiley:00145793:media:feb20014579395012816:feb20014579395012816-math-si1"/>). Peroxynitrite did not increase 8‐oxoguanine levels in DNA.</jats:p>