Debcl, a Proapoptotic Bcl-2 Homologue, Is a Component of the <i>Drosophila melanogaster</i> Cell Death Machinery

DOI PDF 被引用文献9件
  • Paul A. Colussi
    aThe Hanson Centre for Cancer Research, Institute of Medical and Veterinary Science, Adelaide, SA 5000, Australia
  • Leonie M. Quinn
    bDepartment of Genetics, The University of Adelaide, Adelaide, SA 5001, Australia
  • David C.S. Huang
    cThe Walter and Eliza Hall Institute of Medical Research, The Royal Melbourne Hospital, Melbourne, Vic 3050, Australia
  • Michelle Coombe
    bDepartment of Genetics, The University of Adelaide, Adelaide, SA 5001, Australia
  • Stuart H. Read
    aThe Hanson Centre for Cancer Research, Institute of Medical and Veterinary Science, Adelaide, SA 5000, Australia
  • Helena Richardson
    bDepartment of Genetics, The University of Adelaide, Adelaide, SA 5001, Australia
  • Sharad Kumar
    aThe Hanson Centre for Cancer Research, Institute of Medical and Veterinary Science, Adelaide, SA 5000, Australia

書誌事項

公開日
2000-02-21
DOI
  • 10.1083/jcb.148.4.703
公開者
Rockefeller University Press

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説明

<jats:p>Bcl-2 family of proteins are key regulators of apoptosis. Both proapoptotic and antiapoptotic members of this family are found in mammalian cells, but no such proteins have been described in insects. Here, we report the identification and characterization of Debcl, the first Bcl-2 homologue in Drosophila melanogaster. Structurally, Debcl is similar to Bax-like proapoptotic Bcl-2 family members. Ectopic expression of Debcl in cultured cells and in transgenic flies causes apoptosis, which is inhibited by coexpression of the baculovirus caspase inhibitor P35, indicating that Debcl is a proapoptotic protein that functions in a caspase-dependent manner. debcl expression correlates with developmental cell death in specific Drosophila tissues. We also show that debcl genetically interacts with diap1 and dark, and that debcl-mediated apoptosis is not affected by gene dosage of rpr, hid, and grim. Biochemically, Debcl can interact with several mammalian and viral prosurvival Bcl-2 family members, but not with the proapoptotic members, suggesting that it may regulate apoptosis by antagonizing prosurvival Bcl-2 proteins. RNA interference studies indicate that Debcl is required for developmental apoptosis in Drosophila embryos. These results suggest that the main components of the mammalian apoptosis machinery are conserved in insects.</jats:p>

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