Chemical Synthesis of Diubiquitin‐Based Photoaffinity Probes for Selectively Profiling Ubiquitin‐Binding Proteins

Jun Liang, Lin Zhang, Xiang‐Long Tan, Yun‐Kun Qi, Shan Feng, Haiteng Deng, Yijing Yan, Ji‐Shen Zheng, Lei Liu, Chang‐Lin Tian

doi:10.1002/anie.201611659

Chemical Synthesis of Diubiquitin‐Based Photoaffinity Probes for Selectively Profiling Ubiquitin‐Binding Proteins

DOI Web Site Web Site 被引用文献4件

Jun Liang

Hefei National Laboratory for Physical Sciences at the Microscale University of Science and University of Science and Technology of China Hefei 230026 China
Lin Zhang

MOE Key Laboratory of Bioinformatics, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences Tsinghua University Beijing 100084 China
Xiang‐Long Tan

Department of Chemistry Tsinghua University Beijing 100084 China
Yun‐Kun Qi

Department of Chemistry Tsinghua University Beijing 100084 China
Shan Feng

MOE Key Laboratory of Bioinformatics, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences Tsinghua University Beijing 100084 China
Haiteng Deng

MOE Key Laboratory of Bioinformatics, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences Tsinghua University Beijing 100084 China
Yijing Yan

Hefei National Laboratory for Physical Sciences at the Microscale University of Science and University of Science and Technology of China Hefei 230026 China
Ji‐Shen Zheng

Hefei National Laboratory for Physical Sciences at the Microscale University of Science and University of Science and Technology of China Hefei 230026 China
Lei Liu

Department of Chemistry Tsinghua University Beijing 100084 China
Chang‐Lin Tian

Hefei National Laboratory for Physical Sciences at the Microscale University of Science and University of Science and Technology of China Hefei 230026 China

書誌事項

公開日: 2017-02

権利情報

http://onlinelibrary.wiley.com/termsAndConditions#vor

DOI

10.1002/anie.201611659

公開者: Wiley

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説明

<jats:title>Abstract</jats:title><jats:p>Biochemical studies of cellular processes involving polyubiquitin have gained increasing attention. More tools are needed to identify ubiquitin (Ub)‐binding proteins. We report diazirine‐based photoaffinity probes that can capture Ub‐binding proteins in cell lysates, and show that diazirines are preferable to aryl azides as the photo‐crosslinking group, since they decrease non‐selective capture. Photoaffinity probes containing at least two Ub units were required to effectively capture Ub‐binding proteins. Different capture selectivity was observed for probes containing diubiquitin moieties with different types of linkages, thus indicating the potential to develop linkage‐dependent probes for selectively profiling Ub‐binding proteins under various cellular conditions.</jats:p>