Large-Scale Discovery of Induced Point Mutations With High-Throughput TILLING

DOI Web Site 被引用文献30件

書誌事項

公開日
2003-03-01
DOI
  • 10.1101/gr.977903
公開者
Cold Spring Harbor Laboratory

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説明

<jats:p>TILLING (<jats:underline>T</jats:underline>argeting <jats:underline>I</jats:underline>nduced <jats:underline>L</jats:underline>ocal<jats:underline>L</jats:underline>esions <jats:underline>in</jats:underline><jats:underline>G</jats:underline>enomes) is a general reverse-genetic strategy that provides an allelic series of induced point mutations in genes of interest. High-throughput TILLING allows the rapid and low-cost discovery of induced point mutations in populations of chemically mutagenized individuals. As chemical mutagenesis is widely applicable and mutation detection for TILLING is dependent only on sufficient yield of PCR products, TILLING can be applied to most organisms. We have developed TILLING as a service to the <jats:italic>Arabidopsis</jats:italic> community known as the <jats:italic>Arabidopsis</jats:italic>TILLING Project (ATP). Our goal is to rapidly deliver allelic series of ethylmethanesulfonate-induced mutations in target 1-kb loci requested by the international research community. In the first year of public operation, ATP has discovered, sequenced, and delivered >1000 mutations in >100 genes ordered by <jats:italic>Arabidopsis</jats:italic> researchers. The tools and methodologies described here can be adapted to create similar facilities for other organisms.</jats:p>

収録刊行物

  • Genome Research

    Genome Research 13 (3), 524-530, 2003-03-01

    Cold Spring Harbor Laboratory

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