Zinc-Chelated Poly(1-vinylimidazole) and a Carbohydrate Ligand Polycation Form DNA Ternary Complexes for Gene Delivery
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- Shoichiro Asayama
- Department of Applied Chemistry, Tokyo Metropolitan University, 1-1 minami-Osawa, Hachioji, Tokyo 192-0397, Japan
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- Satoshi Nishinohara
- Department of Applied Chemistry, Tokyo Metropolitan University, 1-1 minami-Osawa, Hachioji, Tokyo 192-0397, Japan
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- Hiroyoshi Kawakami
- Department of Applied Chemistry, Tokyo Metropolitan University, 1-1 minami-Osawa, Hachioji, Tokyo 192-0397, Japan
書誌事項
- 公開日
- 2011-08-25
- DOI
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- 10.1021/bc2003378
- 公開者
- American Chemical Society (ACS)
この論文をさがす
説明
Zinc-chelated poly(1-vinylimidazole) (PVIm-Zn) and a carbohydrate ligand polycation, a poly(l-lysine) conjugated with lactose molecules (PLL-Lac), have formed DNA ternary complexes for gene delivery. The particle size of the PVIm-Zn/DNA complexes with negative zeta potential was decreased by the addition of the PLL-Lac. The resulting PLL-Lac/PVIm-Zn/DNA ternary complexes, which exhibited the pH-dependent dissociation of the PLL-Lac, mediated more gene expression than the PVIm/DNA binary complexes. The PLL-Lac/PVIm-Zn/DNA complexes with the specific recognition of cell surface receptors mediated the highest gene expression without cytotoxicity at a relatively lower charge ratio (positive/negative = 2.5). These results suggest that the pH-dependent dissociation of the carbohydrate ligands after the recognition of cell surface receptors, including the physicochemical and biochemical function of PVIm-Zn, played an important role in gene expression.
収録刊行物
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- Bioconjugate Chemistry
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Bioconjugate Chemistry 22 (9), 1864-1868, 2011-08-25
American Chemical Society (ACS)
