Isolation and Characterization of a New
            <i>Clostridium</i>
            sp. That Performs Effective Cellulosic Waste Digestion in a Thermophilic Methanogenic Bioreactor

Hatsumi Shiratori, Hironori Ikeno, Shohei Ayame, Naoaki Kataoka, Akiko Miya, Kuniaki Hosono, Teruhiko Beppu, Kenji Ueda

doi:10.1128/aem.72.5.3702-3709.2006

<jats:title>ABSTRACT</jats:title> <jats:p> A methanogenic bioreactor that utilized wastepaper was developed and operated at 55°C. Microbial community structure analysis showed the presence of a group of clostridia that specifically occurred during the period of high fermentation efficiency. To isolate the effective cellulose digester, the sludge that exhibited high fermentation efficiency was inoculated into a synthetic medium that contained cellulose powder as the sole carbon source and was successively cultivated. A comprehensive 16S rRNA gene sequencing study revealed that the enriched culture contained various clostridia that had diverse phylogenetic positions. The microorganisms were further enriched by successive cultivation with filter paper as the substrate, as well as the bait carrier. A resultant isolate, strain EBR45 (= <jats:italic>Clostridium</jats:italic> sp. strain NBRC101661), was a new member of the order <jats:italic>Clostridiales</jats:italic> phylogenetically and physiologically related to <jats:italic>Clostridium thermocellum</jats:italic> and <jats:italic>Clostridium straminisolvens</jats:italic> . Specific PCR-based monitoring demonstrated that strain EBR45 specifically occurred during the high fermentation efficiency period in the original methanogenic sludge. Strain EBR45 effectively digested office paper in its pure cultivation system with a synthetic medium. </jats:p>

Isolation and Characterization of a New <i>Clostridium</i> sp. That Performs Effective Cellulosic Waste Digestion in a Thermophilic Methanogenic Bioreactor

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