A point mutation in a silencer module reduces the promoter activity for the human mercaptopyruvate sulfurtransferase
書誌事項
- 公開日
- 2004-11
- DOI
-
- 10.1016/j.bbaexp.2004.09.007
- 公開者
- Elsevier BV
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説明
A promoter region of human mercaptopyruvate sulfurtransferase (MST) [EC 2.8.1.2] is G+C-rich and TATA-less, showing features of a house-keeping gene. In the core promoter, a GC box (-284:GGGGCGTGGC:-275) and an initiator (-219:TTATATG:-225) are found. A cap site hunting analysis for human liver cDNA revealed four possible transcriptional start sites, nucleotides -223, -159, -35 and -25. Point mutagenesis and deletion studies suggest that a module of the silencer element is -394:GCTG:-391. A replacement of -391G to C lost the silencer function; on the other hand, a replacement of -394G to T or C, -393C to T or -392T to G markedly reduced the promoter activity.
収録刊行物
-
- Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression
-
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression 1680 (3), 176-184, 2004-11
Elsevier BV
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キーワード
- Binding Sites
- DNA, Complementary
- Base Sequence
- Molecular Sequence Data
- Gene Expression Regulation, Enzymologic
- GC Rich Sequence
- Enhancer Elements, Genetic
- Liver
- Sequence Homology, Nucleic Acid
- Sulfurtransferases
- Silencer Elements, Transcriptional
- Humans
- Point Mutation
- Cloning, Molecular
- Transcription Initiation Site
- Promoter Regions, Genetic
- Sequence Deletion
