Highly Stable Double‐Stranded DNA Containing Sequential Silver(I)‐Mediated 7‐Deazaadenine/Thymine Watson–Crick Base Pairs
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- Noelia Santamaría‐Díaz
- Facultad de Ciencias Departamento Química Inorgánica Universidad de Granada Avd. Fuentenueva s/n 18071 Granada Spain
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- José M. Méndez‐Arriaga
- Facultad de Ciencias Departamento Química Inorgánica Universidad de Granada Avd. Fuentenueva s/n 18071 Granada Spain
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- Juan M. Salas
- Facultad de Ciencias Departamento Química Inorgánica Universidad de Granada Avd. Fuentenueva s/n 18071 Granada Spain
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- Miguel A. Galindo
- Facultad de Ciencias Departamento Química Inorgánica Universidad de Granada Avd. Fuentenueva s/n 18071 Granada Spain
書誌事項
- 公開日
- 2016-03-23
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1002/anie.201600924
- 公開者
- Wiley
この論文をさがす
説明
<jats:title>Abstract</jats:title><jats:p>The oligonucleotide d(TX)<jats:sub>9</jats:sub>, which consists of an octadecamer sequence with alternating non‐canonical 7‐deazaadenine (X) and canonical thymine (T) as the nucleobases, was synthesized and shown to hybridize into double‐stranded DNA through the formation of hydrogen‐bonded Watson–Crick base pairs. dsDNA with metal‐mediated base pairs was then obtained by selectively replacing W‐C hydrogen bonds by coordination bonds to central silver(I) ions. The oligonucleotide <jats:bold>I</jats:bold> adopts a duplex structure in the absence of Ag<jats:sup>+</jats:sup> ions, and its stability is significantly enhanced in the presence of Ag<jats:sup>+</jats:sup> ions while its double‐helix structure is retained. Temperature‐dependent UV spectroscopy, circular dichroism spectroscopy, and ESI mass spectrometry were used to confirm the selective formation of the silver(I)‐mediated base pairs. This strategy could become useful for preparing stable metallo‐DNA‐based nanostructures.</jats:p>
収録刊行物
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- Angewandte Chemie International Edition
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Angewandte Chemie International Edition 55 (21), 6170-6174, 2016-03-23
Wiley
