Evidence for the presence of multiple forms of Sph kinase in human platelets
-
- Yoshiko BANNO
- Department of Biochemistry, Gifu University School of Medicine, Tsukasamachi-40, Gifu 500-8705, Japan
-
- Makiko KATO
- Laboratory of Biochemistry, Gifu Pharmaceutical University, Mitahora-higashi, Gifu 502-0003, Japan
-
- Akira HARA
- Laboratory of Biochemistry, Gifu Pharmaceutical University, Mitahora-higashi, Gifu 502-0003, Japan
-
- Yoshinori NOZAWA
- Department of Biochemistry, Gifu University School of Medicine, Tsukasamachi-40, Gifu 500-8705, Japan
書誌事項
- 公開日
- 1998-10-15
- DOI
-
- 10.1042/bj3350301
- 公開者
- Portland Press Ltd.
この論文をさがす
説明
<jats:p>The intracellular distribution of sphingosine (Sph) kinase activity was examined in human platelets. A large proportion (72%) of the total activity was found to be associated with the membrane fraction, and the membrane-associated fraction had higher specific activity compared with the cytosolic enzyme. Most of the membrane-associated activity could be extracted with 1 M NaCl. The cytosolic activity was unstable upon heat treatment, with 80% of the activity being lost during incubation at 45 °C for 1 h, whereas the NaCl-extractable fraction was stable under the same conditions. When subjected to Mono Q column chromatography, the cytosolic fraction produced two activity peaks and the NaCl-extractable fraction gave a single peak. These three Sph kinase activities showed different responses to stimulation by β-octylglucoside and inhibition by N,N-dimethylsphingosine and l-threo-dihydrosphingosine, suggesting the presence of multiple enzyme forms in human platelets.</jats:p>
収録刊行物
-
- Biochemical Journal
-
Biochemical Journal 335 (2), 301-304, 1998-10-15
Portland Press Ltd.
