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- Erin E. Dymek
- Department of Biological Sciences, Dartmouth College, Hanover, NH 03755
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- Thomas Heuser
- Department of Biology, Brandeis University, Waltham, MA 02453
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- Daniela Nicastro
- Department of Biology, Brandeis University, Waltham, MA 02453
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- Elizabeth F. Smith
- Department of Biological Sciences, Dartmouth College, Hanover, NH 03755
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- Erika L. F. Holzbaur
- editor
書誌事項
- 公開日
- 2011-07-15
- DOI
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- 10.1091/mbc.e11-03-0271
- 公開者
- American Society for Cell Biology (ASCB)
この論文をさがす
説明
<jats:p>The ubiquitous calcium binding protein, calmodulin (CaM), plays a major role in regulating the motility of all eukaryotic cilia and flagella. We previously identified a CaM and Spoke associated Complex (CSC) and provided evidence that this complex mediates regulatory signals between the radial spokes and dynein arms. We have now used an artificial microRNA (amiRNA) approach to reduce expression of two CSC subunits in Chlamydomonas. For all amiRNA mutants, the entire CSC is lacking or severely reduced in flagella. Structural studies of mutant axonemes revealed that assembly of radial spoke 2 is defective. Furthermore, analysis of both flagellar beating and microtubule sliding in vitro demonstrates that the CSC plays a critical role in modulating dynein activity. Our results not only indicate that the CSC is required for spoke assembly and wild-type motility, but also provide evidence for heterogeneity among the radial spokes.</jats:p>
収録刊行物
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- Molecular Biology of the Cell
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Molecular Biology of the Cell 22 (14), 2520-2531, 2011-07-15
American Society for Cell Biology (ASCB)
