<i>PSF1</i> Is Essential for Early Embryogenesis in Mice
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- Masaya Ueno
- Department of Stem Cell Biology, Cancer Research Institute, Kanazawa University
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- Machiko Itoh
- Department of Stem Cell Biology, Cancer Research Institute, Kanazawa University
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- Lingyu Kong
- Department of Stem Cell Biology, Cancer Research Institute, Kanazawa University
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- Kazushi Sugihara
- Institute for Experimental Animals, Kanazawa University Advanced Science Research Center, Kanazawa, Japan
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- Masahide Asano
- Institute for Experimental Animals, Kanazawa University Advanced Science Research Center, Kanazawa, Japan
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- Nobuyuki Takakura
- Department of Stem Cell Biology, Cancer Research Institute, Kanazawa University
書誌事項
- タイトル別名
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- PSF1 is essential for early embryogenesis in mice.
- 公開日
- 2005-12-01
- 資源種別
- journal article
- 権利情報
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- https://journals.asm.org/non-commercial-tdm-license
- DOI
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- 10.1128/mcb.25.23.10528-10532.2005
- 公開者
- Informa UK Limited
この論文をさがす
説明
Psf1 (partner of sld five 1) forms a novel heterotetramer complex, GINS (Go, Ichi, Nii, and San; five, one, two, and three, respectively, in Japanese), with Sld5, Psf2, and Psf3. The formation of this complex is essential for the initiation of DNA replication in yeast and Xenopus laevis egg extracts. Although all of the components are well conserved in higher eukaryotes, the biological function in vivo is largely unknown. We originally cloned the mouse ortholog of PSF1 from a hematopoietic stem cell cDNA library and found that PSF1 is expressed in blastocysts, adult bone marrow, and testis, in which the stem cell system is active. Here we used the gene-targeting technique to determine the physiological function of PSF1 in vivo. Mice homozygous for a nonfunctional mutant of PSF1 died in utero around the time of implantation. PSF1-/- blastocysts failed to show outgrowth in culture and exhibited a cell proliferation defect. Our data clearly indicate that PSF1 is required for early embryogenesis.
収録刊行物
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- Molecular and Cellular Biology
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Molecular and Cellular Biology 25 (23), 10528-10532, 2005-12-01
Informa UK Limited
関連未分類成果物
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キーワード
- Mice, Knockout
- Genes, Essential
- Time Factors
- Embryonic Development
- Gene Expression Regulation, Developmental
- Mice, Inbred C57BL
- Mice
- Blastocyst
- Embryo Loss
- Animals
- ATP-Binding Cassette Transporters
- ATP Binding Cassette Transporter, Subfamily B, Member 2
- Cloning, Molecular
- Gene Deletion
- Cell Proliferation
詳細情報 詳細情報について
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- CRID
- 1361699995657500800
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- NII論文ID
- 30021004243
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- ISSN
- 10985549
- 02707306
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- PubMed
- 16287864
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- 資料種別
- journal article
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- データソース種別
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- Crossref
- PubMed
- CiNii Articles
- OpenAIRE
- IRDB
