Mode of Interaction of Amphiphilic α-Helical Peptide with Phosphatidylcholines at the Air−Water Interface
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- Hiromichi Nakahara
- Division of Biointerfacial Science, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; Department of Chemistry, Graduate School of Science, Kyushu University, 6-1-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan; and Department of Chemistry, Faculty of Science, Fukuoka University, 8-19-1 Nanakuma, Johnan-ku, Fukuoka 814-0180, Japan
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- Shohei Nakamura
- Division of Biointerfacial Science, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; Department of Chemistry, Graduate School of Science, Kyushu University, 6-1-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan; and Department of Chemistry, Faculty of Science, Fukuoka University, 8-19-1 Nanakuma, Johnan-ku, Fukuoka 814-0180, Japan
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- Takato Hiranita
- Division of Biointerfacial Science, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; Department of Chemistry, Graduate School of Science, Kyushu University, 6-1-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan; and Department of Chemistry, Faculty of Science, Fukuoka University, 8-19-1 Nanakuma, Johnan-ku, Fukuoka 814-0180, Japan
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- Hideya Kawasaki
- Division of Biointerfacial Science, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; Department of Chemistry, Graduate School of Science, Kyushu University, 6-1-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan; and Department of Chemistry, Faculty of Science, Fukuoka University, 8-19-1 Nanakuma, Johnan-ku, Fukuoka 814-0180, Japan
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- Sannamu Lee
- Division of Biointerfacial Science, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; Department of Chemistry, Graduate School of Science, Kyushu University, 6-1-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan; and Department of Chemistry, Faculty of Science, Fukuoka University, 8-19-1 Nanakuma, Johnan-ku, Fukuoka 814-0180, Japan
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- Gohsuke Sugihara
- Division of Biointerfacial Science, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; Department of Chemistry, Graduate School of Science, Kyushu University, 6-1-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan; and Department of Chemistry, Faculty of Science, Fukuoka University, 8-19-1 Nanakuma, Johnan-ku, Fukuoka 814-0180, Japan
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- Osamu Shibata
- Division of Biointerfacial Science, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; Department of Chemistry, Graduate School of Science, Kyushu University, 6-1-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan; and Department of Chemistry, Faculty of Science, Fukuoka University, 8-19-1 Nanakuma, Johnan-ku, Fukuoka 814-0180, Japan
書誌事項
- 公開日
- 2005-12-24
- DOI
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- 10.1021/la0524925
- 公開者
- American Chemical Society (ACS)
この論文をさがす
説明
Surface pressure (pi)-, surface potential (deltaV)-, and dipole moment (mu(perpendicular))-area (A) isotherms and morphological behavior were examined for monolayers of a newly designed 18-mer amphiphilic alpha-helical peptide (Hel 13-5), DPPC, and DPPC/egg-PC (1:1) and their combinations by the Wilhelmy method, ionizing electrode method, fluorescence microscopy (FM), and atomic force microscopy (AFM). The newly designed Hel 13-5 showed rapid adsorption into the air-liquid interface to form interfacial films such as a SP-B function. Regardless of the composition and constituents in their multicomponent system of DPPC/egg-PC, the collapse pressure (pi(c); approximately 42 mN m(-1)) was constant, implying that Hel 13-5 with the fluid composition of egg-PC is squeezed out of Hel 13-5/DPPC/egg-PC monolayers accompanying a two- to three-dimensional phase transformation. FM showed that adding a small amount of Hel 13-5 to DPPC induced a dispersed pattern of ordered domains with a "moth-eaten" appearance, whereas shrinkage of ordered domains in size occurred for the DPPC/egg-PC mixture with Hel 13-5. Furthermore, AFM indicated that (i) the intermediate phase was formed in pure Hel 13-5 systems between monolayer states and excluded nanoparticles, (ii) protrusions necessarily located on DPPC monolayers, and (iii) beyond the collapse pressure of Hel 13-5, Hel 13-5 was squeezed out of the system into the aqueous subphase. Furthermore, hysteresis curves of these systems nicely resemble those of the DPPC/SP-B and DPPC/SP-C mixtures reported before.
収録刊行物
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- Langmuir
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Langmuir 22 (3), 1182-1192, 2005-12-24
American Chemical Society (ACS)
