Bioreactor-based bone tissue engineering: The influence of dynamic flow on osteoblast phenotypic expression and matrix mineralization

DOI Web Site 被引用文献12件
  • Xiaojun Yu
    Departments of Orthopaedic Surgery, Biomedical Engineering, and Chemical Engineering, University of Virginia, Charlottesville, VA 22903; The Wistar Institute, Philadelphia, PA 19104; and Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104
  • Edward A. Botchwey
    Departments of Orthopaedic Surgery, Biomedical Engineering, and Chemical Engineering, University of Virginia, Charlottesville, VA 22903; The Wistar Institute, Philadelphia, PA 19104; and Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104
  • Elliot M. Levine
    Departments of Orthopaedic Surgery, Biomedical Engineering, and Chemical Engineering, University of Virginia, Charlottesville, VA 22903; The Wistar Institute, Philadelphia, PA 19104; and Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104
  • Solomon R. Pollack
    Departments of Orthopaedic Surgery, Biomedical Engineering, and Chemical Engineering, University of Virginia, Charlottesville, VA 22903; The Wistar Institute, Philadelphia, PA 19104; and Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104
  • Cato T. Laurencin
    Departments of Orthopaedic Surgery, Biomedical Engineering, and Chemical Engineering, University of Virginia, Charlottesville, VA 22903; The Wistar Institute, Philadelphia, PA 19104; and Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104

書誌事項

公開日
2004-07-26
DOI
  • 10.1073/pnas.0402532101
公開者
Proceedings of the National Academy of Sciences

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説明

<jats:p> An important issue in tissue engineering concerns the possibility of limited tissue ingrowth in tissue-engineered constructs because of insufficient nutrient transport. We report a dynamic flow culture system using high-aspect-ratio vessel rotating bioreactors and 3D scaffolds for culturing rat calvarial osteoblast cells. 3D scaffolds were designed by mixing lighter-than-water (density, <1g/ml) and heavier-than-water (density, >1g/ml) microspheres of 85:15 poly(lactide- <jats:italic>co</jats:italic> -glycolide). We quantified the rate of 3D flow through the scaffolds by using a particle-tracking system, and the results suggest that motion trajectories and, therefore, the flow velocity around and through scaffolds in rotating bioreactors can be manipulated by varying the ratio of heavier-than-water to lighter-than-water microspheres. When rat primary calvarial cells were cultured on the scaffolds in bioreactors for 7 days, the 3D dynamic flow environment affected bone cell distribution and enhanced cell phenotypic expression and mineralized matrix synthesis within tissue-engineered constructs compared with static conditions. These studies provide a foundation for exploring the effects of dynamic flow on osteoblast function and provide important insight into the design and optimization of 3D scaffolds suitable in bioreactors for <jats:italic>in vitro</jats:italic> tissue engineering of bone. </jats:p>

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