A Genetic Analysis of Glucocorticoid Receptor Signaling: Identification and Characterization of Ligand-Effect Modulators in <i>Saccharomyces cerevisiae</i>

Raquel Sitcheran, Roger Emter, Anastasia Kralli, Keith R Yamamoto

doi:10.1093/genetics/156.3.963

A Genetic Analysis of Glucocorticoid Receptor Signaling: Identification and Characterization of Ligand-Effect Modulators in <i>Saccharomyces cerevisiae</i>

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Raquel Sitcheran

Department of Cellular and Molecular Pharmacology, University of California, San Francisco, California 91413-0450
Roger Emter

Division of Biochemistry, Biozentrum, University of Basel, Basel CH4056, Switzerland
Anastasia Kralli

Division of Biochemistry, Biozentrum, University of Basel, Basel CH4056, Switzerland
Keith R Yamamoto

Department of Cellular and Molecular Pharmacology, University of California, San Francisco, California 91413-0450

書誌事項

公開日: 2000-11-01

権利情報

https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model

DOI

10.1093/genetics/156.3.963

公開者: Oxford University Press (OUP)

説明

<jats:title>Abstract</jats:title> <jats:p>To find novel components in the glucocorticoid signal transduction pathway, we performed a yeast genetic screen to identify ligand-effect modulators (LEMs), proteins that modulate the cellular response to hormone. We isolated several mutants that conferred increased glucocorticoid receptor (GR) activity in response to dexamethasone and analyzed two of them in detail. These studies identify two genes, LEM3 and LEM4, which correspond to YNL323w and ERG6, respectively. LEM3 is a putative transmembrane protein of unknown function, and ERG6 is a methyltransferase in the ergosterol biosynthetic pathway. Analysis of null mutants indicates that LEM3 and ERG6 act at different steps in the GR signal transduction pathway.</jats:p>