Development of phase plates for electron microscopes and their biological application
書誌事項
- 公開日
- 2008-02-08
- 権利情報
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- http://www.springer.com/tdm
- DOI
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- 10.1007/s00249-008-0264-5
- 公開者
- Springer Science and Business Media LLC
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説明
After slow progress in the efforts to develop phase plates for electron microscopes, functional phase plates with thin carbon films have recently been reported. An electron microscope enhanced with thin-film phase plates has practical advantages. It permits collecting high-contrast images of intact biological specimens without harsh and lengthy sample preparation, such as fixation, dehydration, resin-embedding, staining and thin-sectioning. This report reviews the state of the art for phase plates in biological electron microscopy and focuses upon the conditions required for functional thin-film phase plates. The current disadvantages of thin-film phase plates are also addressed and potential solutions are proposed.
収録刊行物
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- European Biophysics Journal
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European Biophysics Journal 37 (4), 345-358, 2008-02-08
Springer Science and Business Media LLC
