Establishment and characterization of an erythropoietin-dependent subline, UT-7/Epo, derived from human leukemia cell line, UT-7
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- N Komatsu
- Department of Medicine, Jichi Medical School, Tochigi, Japan.
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- M Yamamoto
- Department of Medicine, Jichi Medical School, Tochigi, Japan.
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- H Fujita
- Department of Medicine, Jichi Medical School, Tochigi, Japan.
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- A Miwa
- Department of Medicine, Jichi Medical School, Tochigi, Japan.
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- K Hatake
- Department of Medicine, Jichi Medical School, Tochigi, Japan.
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- T Endo
- Department of Medicine, Jichi Medical School, Tochigi, Japan.
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- H Okano
- Department of Medicine, Jichi Medical School, Tochigi, Japan.
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- T Katsube
- Department of Medicine, Jichi Medical School, Tochigi, Japan.
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- Y Fukumaki
- Department of Medicine, Jichi Medical School, Tochigi, Japan.
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- S Sassa
- Department of Medicine, Jichi Medical School, Tochigi, Japan.
書誌事項
- 公開日
- 1993-07-15
- DOI
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- 10.1182/blood.v82.2.456.456
- 公開者
- American Society of Hematology
この論文をさがす
説明
<jats:title>Abstract</jats:title> <jats:p>UT-7 is a human leukemic cell line capable of growing in interleukin-3 (IL-3), granulocyte/macrophage colony-stimulating factor (GM-CSF), or erythropoietin (Epo) (Komatsu et al, Cancer Res 51:341, 1991). To study the effect of Epo on proliferation and differentiation of UT-7, we maintained the UT-7 cell culture for more than 6 months in the presence of Epo. As a result, a subline, UT-7/Epo, was established. The growth of UT-7/Epo could be supported by Epo but not by GM-CSF or IL-3. UT- 7/Epo showed a greater level of heme content and ratio of benzidine- positive staining cells than did UT-7. Butyric acid promoted the synthesis of hemoglobin in UT-7/Epo, but not UT-7. Further, the mRNA concentrations of the c-myb oncogene and GM-CSF receptor beta-subunit were decreased substantially in UT-7/Epo cells. These findings showed that UT-7/Epo cells had progressed further in erythroid development than UT-7 cells, and suggested that long-term culture in Epo had promoted this differentiation. Whereas availability of the Epo receptor (Epo-R) for binding of Epo was reduced in UT-7/Epo cells compared with UT-7 cells, the Epo-R showed a similar affinity for Epo. This observation suggested that change(s) in postreceptor signaling step might be involved in the establishment and maintenance of the UT-7/Epo phenotype.</jats:p>
収録刊行物
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- Blood
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Blood 82 (2), 456-464, 1993-07-15
American Society of Hematology
