Comparative Analysis of Extended-Spectrum-β-Lactamase CTX-M-65-Producing Salmonella enterica Serovar Infantis Isolates from Humans, Food Animals, and Retail Chickens in the United States

DOI Web Site 被引用文献1件
  • Heather Tate
    Center for Veterinary Medicine, U.S. Food and Drug Administration, Silver Spring, Maryland, USA
  • Jason P. Folster
    National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
  • Chih-Hao Hsu
    Center for Veterinary Medicine, U.S. Food and Drug Administration, Silver Spring, Maryland, USA
  • Jessica Chen
    National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
  • Maria Hoffmann
    Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, Silver Spring, Maryland, USA
  • Cong Li
    Center for Veterinary Medicine, U.S. Food and Drug Administration, Silver Spring, Maryland, USA
  • Cesar Morales
    Food Safety Inspection Service, U.S. Department of Agriculture, Washington, DC, USA
  • Gregory H. Tyson
    Center for Veterinary Medicine, U.S. Food and Drug Administration, Silver Spring, Maryland, USA
  • Sampa Mukherjee
    Center for Veterinary Medicine, U.S. Food and Drug Administration, Silver Spring, Maryland, USA
  • Allison C. Brown
    National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
  • Alice Green
    Food Safety Inspection Service, U.S. Department of Agriculture, Washington, DC, USA
  • Wanda Wilson
    Food Safety Inspection Service, U.S. Department of Agriculture, Washington, DC, USA
  • Uday Dessai
    Food Safety Inspection Service, U.S. Department of Agriculture, Washington, DC, USA
  • Jason Abbott
    Center for Veterinary Medicine, U.S. Food and Drug Administration, Silver Spring, Maryland, USA
  • Lavin Joseph
    National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
  • Jovita Haro
    Food Safety Inspection Service, U.S. Department of Agriculture, Washington, DC, USA
  • Sherry Ayers
    Center for Veterinary Medicine, U.S. Food and Drug Administration, Silver Spring, Maryland, USA
  • Patrick F. McDermott
    Center for Veterinary Medicine, U.S. Food and Drug Administration, Silver Spring, Maryland, USA
  • Shaohua Zhao
    Center for Veterinary Medicine, U.S. Food and Drug Administration, Silver Spring, Maryland, USA

抄録

<jats:title>ABSTRACT</jats:title> <jats:p> We sequenced the genomes of 10 <jats:named-content content-type="genus-species">Salmonella enterica</jats:named-content> serovar Infantis isolates containing <jats:italic>bla</jats:italic> <jats:sub>CTX-M-65</jats:sub> obtained from chicken, cattle, and human sources collected between 2012 and 2015 in the United States through routine National Antimicrobial Resistance Monitoring System (NARMS) surveillance and product sampling programs. We also completely assembled the plasmids from four of the isolates. All isolates had a D87Y mutation in the <jats:italic>gyrA</jats:italic> gene and harbored between 7 and 10 resistance genes [ <jats:italic>aph(4)-Ia</jats:italic> , <jats:italic>aac(3)-IVa</jats:italic> , <jats:italic>aph(3</jats:italic> ′ <jats:italic>)-Ic</jats:italic> , <jats:italic>bla</jats:italic> <jats:sub>CTX-M-65</jats:sub> , <jats:italic>fosA3</jats:italic> , <jats:italic>floR</jats:italic> , <jats:italic>dfrA14</jats:italic> , <jats:italic>sul1</jats:italic> , <jats:italic>tetA</jats:italic> , <jats:italic>aadA1</jats:italic> ] located in two distinct sites of a megaplasmid (∼316 to 323 kb) similar to that described in a <jats:italic>bla</jats:italic> <jats:sub>CTX-M-65</jats:sub> -positive <jats:italic>S</jats:italic> . Infantis isolate from a patient in Italy. High-quality single nucleotide polymorphism (hqSNP) analysis revealed that all U.S. isolates were closely related, separated by only 1 to 38 pairwise high-quality SNPs, indicating a high likelihood that strains from humans, chickens, and cattle recently evolved from a common ancestor. The U.S. isolates were genetically similar to the <jats:italic>bla</jats:italic> <jats:sub>CTX-M-65</jats:sub> -positive <jats:italic>S</jats:italic> . Infantis isolate from Italy, with a separation of 34 to 47 SNPs. This is the first report of the <jats:italic>bla</jats:italic> <jats:sub>CTX-M-65</jats:sub> gene and the pESI (plasmid for emerging <jats:italic>S</jats:italic> . Infantis)-like megaplasmid from <jats:italic>S</jats:italic> . Infantis in the United States, and it illustrates the importance of applying a global One Health human and animal perspective to combat antimicrobial resistance. </jats:p>

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