Characterization of a male specific region containing a candidate sex determining gene in Atlantic cod
書誌事項
- 公開日
- 2019-01-15
- 権利情報
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- https://creativecommons.org/licenses/by/4.0
- https://creativecommons.org/licenses/by/4.0
- DOI
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- 10.1038/s41598-018-36748-8
- 公開者
- Springer Science and Business Media LLC
説明
<jats:title>Abstract</jats:title><jats:p>The genetic mechanisms determining sex in teleost fishes are highly variable and the master sex determining gene has only been identified in few species. Here we characterize a male-specific region of 9 kb on linkage group 11 in Atlantic cod (<jats:italic>Gadus morhua</jats:italic>) harboring a single gene named<jats:italic>zkY</jats:italic>for zinc knuckle on the Y chromosome. Diagnostic PCR test of phenotypically sexed males and females confirm the sex-specific nature of the Y-sequence. We identified twelve highly similar autosomal gene copies of<jats:italic>zkY</jats:italic>, of which eight code for proteins containing the zinc knuckle motif. 3D modeling suggests that the amino acid changes observed in six copies might influence the putative RNA-binding specificity. Cod zkY and the autosomal proteins zk1 and zk2 possess an identical zinc knuckle structure, but only the Y-specific gene<jats:italic>zkY</jats:italic>was expressed at high levels in the developing larvae before the onset of sex differentiation. Collectively these data suggest<jats:italic>zkY</jats:italic>as a candidate master masculinization gene in Atlantic cod. PCR amplification of Y-sequences in Arctic cod (<jats:italic>Arctogadus glacialis</jats:italic>) and Greenland cod (<jats:italic>Gadus macrocephalus ogac</jats:italic>) suggests that the male-specific region emerged in codfishes more than 7.5 million years ago.</jats:p>
収録刊行物
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- Scientific Reports
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Scientific Reports 9 (1), 2019-01-15
Springer Science and Business Media LLC
