AKAP220 manages apical actin networks that coordinate aquaporin-2 location and renal water reabsorption
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- Jennifer L. Whiting
- Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195;
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- Leah Ogier
- Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195;
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- Katherine A. Forbush
- Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195;
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- Paula Bucko
- Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195;
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- Janani Gopalan
- Department of Pharmacology, University of Washington, Seattle, WA 98195
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- Ole-Morten Seternes
- Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195;
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- Lorene K. Langeberg
- Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195;
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- John D. Scott
- Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195;
書誌事項
- 公開日
- 2016-07-11
- DOI
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- 10.1073/pnas.1607745113
- 公開者
- Proceedings of the National Academy of Sciences
この論文をさがす
説明
<jats:title>Significance</jats:title> <jats:p>Systemic control of water homeostasis is a vital physiological process. Vasopressin-regulated reabsorption of water through aquaporin-2 (AQP2) water pores in the kidney preserves fluid balance and results in more concentrated urine. We have discovered that the scaffolding protein A-Kinase Anchoring Protein 220 (AKAP220) controls vasopressin-independent aspects of AQP2 trafficking at apical membranes of cells of the kidney-collecting ducts. We postulate that this proceeds via a molecular mechanism that evokes RhoA-mediated modulation of “actin barrier” dynamics. Loss of AKAP220 leads to accumulation of AQP2 at the apical plasma membrane and reduces urine-diluting capacity during overhydration. This phenotype may be clinically relevant, as accumulation of AQP2 at the apical membrane is the desired therapeutic outcome when treating patients with certain renal disorders, including nephrogenic diabetes insipidus.</jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 113 (30), E4328-, 2016-07-11
Proceedings of the National Academy of Sciences
