Cell behaviour on micropatterned substrata: limits of extracellular matrix geometry for spreading and adhesion

DOI PDF 被引用文献10件
  • Dirk Lehnert
    Department of Biology, University of Konstanz, Universitaetstrasse 10, 78457 Konstanz, Germany
  • Bernhard Wehrle-Haller
    Department of Pathology, Centre Medical Universitaire, Geneva, Switzerland
  • Christian David
    Laboratory for Micro- and Nanotechnology, Paul Scherrer Institut, Villigen-PSI, Switzerland
  • Ulrich Weiland
    Department of Biology, University of Konstanz, Universitaetstrasse 10, 78457 Konstanz, Germany
  • Christoph Ballestrem
    Department of Pathology, Centre Medical Universitaire, Geneva, Switzerland
  • Beat A. Imhof
    Department of Pathology, Centre Medical Universitaire, Geneva, Switzerland
  • Martin Bastmeyer
    Department of Biology, University of Konstanz, Universitaetstrasse 10, 78457 Konstanz, Germany

書誌事項

公開日
2004-01-01
DOI
  • 10.1242/jcs.00836
公開者
The Company of Biologists

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説明

<jats:p>Cell adhesion, spreading and migration require the dynamic formation and dispersal of contacts with the extracellular matrix (ECM). In vivo, the number, availability and distribution of ECM binding sites dictate the shape of a cell and determine its mobility. To analyse the geometrical limits of ECM binding sites required for cell attachment and spreading, we used microcontact printing to produce regular patterns of ECM protein dots of defined size separated by nonadhesive regions. Cells cultured on these substrata adhere to and spread on ECM regions as small as 0.1 μm2, when spacing between dots is less than 5 μm. Spacing of 5-25 μm induces a cell to adapt its shape to the ECM pattern. The ability to spread and migrate on dots ≥1 μm2 ceases when the dot separation is ≥30 μm. The extent of cell spreading is directly correlated to the total substratum coverage with ECM-proteins, but irrespective of the geometrical pattern. An optimal spreading extent is reached at a surface coating above 15%. Knowledge of these geometrical limits is essential for an understanding of cell adhesion and migration, and for the design of artificial surfaces that optimally interact with cells in a living tissue.</jats:p>

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