Foxj1 regulates floor plate cilia architecture and modifies the response of cells to sonic hedgehog signalling
-
- Catarina Cruz
- MRC National Institute for Medical Research, Mill Hill, London NW7 1AA, UK.
-
- Vanessa Ribes
- MRC National Institute for Medical Research, Mill Hill, London NW7 1AA, UK.
-
- Eva Kutejova
- MRC National Institute for Medical Research, Mill Hill, London NW7 1AA, UK.
-
- Jordi Cayuso
- Instituto de Biología Molecular de Barcelona, CSIC, Parc Científic de Barcelona, C/Josep Samitier 1-5, Barcelona, 08028, Spain.
-
- Victoria Lawson
- MRC National Institute for Medical Research, Mill Hill, London NW7 1AA, UK.
-
- Dominic Norris
- MRC Mammalian Genetics Unit, Harwell, OX11 0RD, UK.
-
- Jonathan Stevens
- MRC Mammalian Genetics Unit, Harwell, OX11 0RD, UK.
-
- Megan Davey
- Division of Genetics and Genomics, Roslin Institute, Roslin, EH25 9PS, UK.
-
- Ken Blight
- Cancer Research UK London Research Institute, 44 Lincoln's Inn Fields, London WC2A 3PX, UK.
-
- Fiona Bangs
- Biology and Biochemistry Department, University of Bath, Bath BA2 7AY, UK.
-
- Anita Mynett
- MRC National Institute for Medical Research, Mill Hill, London NW7 1AA, UK.
-
- Elizabeth Hirst
- MRC National Institute for Medical Research, Mill Hill, London NW7 1AA, UK.
-
- Rachel Chung
- MRC National Institute for Medical Research, Mill Hill, London NW7 1AA, UK.
-
- Nikolaos Balaskas
- MRC National Institute for Medical Research, Mill Hill, London NW7 1AA, UK.
-
- Steven L. Brody
- Pulmonary and Critical Care Medicine, Department of Internal Medicine, Washington University School of Medicine, St Louis, MO 63110, USA.
-
- Elisa Marti
- Instituto de Biología Molecular de Barcelona, CSIC, Parc Científic de Barcelona, C/Josep Samitier 1-5, Barcelona, 08028, Spain.
-
- James Briscoe
- MRC National Institute for Medical Research, Mill Hill, London NW7 1AA, UK.
書誌事項
- 公開日
- 2010-12-15
- DOI
-
- 10.1242/dev.051714
- 公開者
- The Company of Biologists
この論文をさがす
説明
<jats:p>Sonic hedgehog signalling is essential for the embryonic development of many tissues including the central nervous system, where it controls the pattern of cellular differentiation. A genome-wide screen of neural progenitor cells to evaluate the Shh signalling-regulated transcriptome identified the forkhead transcription factor Foxj1. In both chick and mouse Foxj1 is expressed in the ventral midline of the neural tube in cells that make up the floor plate. Consistent with the role of Foxj1 in the formation of long motile cilia, floor plate cells produce cilia that are longer than the primary cilia found elsewhere in the neural tube, and forced expression of Foxj1 in neuroepithelial cells is sufficient to increase cilia length. In addition, the expression of Foxj1 in the neural tube and in an Shh-responsive cell line attenuates intracellular signalling by decreasing the activity of Gli proteins, the transcriptional mediators of Shh signalling. We show that this function of Foxj1 depends on cilia. Nevertheless, floor plate identity and ciliogenesis are unaffected in mouse embryos lacking Foxj1 and we provide evidence that additional transcription factors expressed in the floor plate share overlapping functions with Foxj1. Together, these findings identify a novel mechanism that modifies the cellular response to Shh signalling and reveal morphological and functional features of the amniote floor plate that distinguish these cells from the rest of the neuroepithelium.</jats:p>
収録刊行物
-
- Development
-
Development 137 (24), 4271-4282, 2010-12-15
The Company of Biologists
