Quantifying <i>E. coli</i> Proteome and Transcriptome with Single-Molecule Sensitivity in Single Cells
-
- Yuichi Taniguchi
- Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.
-
- Paul J. Choi
- Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.
-
- Gene-Wei Li
- Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.
-
- Huiyi Chen
- Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.
-
- Mohan Babu
- Banting and Best Department of Medical Research, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto M5S 3E1, Canada.
-
- Jeremy Hearn
- Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.
-
- Andrew Emili
- Banting and Best Department of Medical Research, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto M5S 3E1, Canada.
-
- X. Sunney Xie
- Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.
説明
<jats:title>Devil in the Detail</jats:title> <jats:p> Genetically identical cells in the same environment can show variation in gene expression that may cause phenotypic variation at the single-cell level. But how noisy are most genes? <jats:bold> Taniguchi <jats:italic>et al.</jats:italic> </jats:bold> (p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" page="533" related-article-type="in-this-issue" vol="329" xlink:href="10.1126/science.1188308">533</jats:related-article> ; see the Perspective by <jats:bold> <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="5991" page="518" related-article-type="in-this-issue" vol="329" xlink:href="10.1126/science.1194036">Tyagi</jats:related-article> </jats:bold> ) now report single-cell global profiling of both messenger RNA (mRNA) and proteins in <jats:italic>Escherichia coli</jats:italic> using a yellow fluorescent protein fusion library. As well as a common extrinsic noise in high-abundance proteins, large fluctuations were observed in low-abundance proteins. Remarkably, in single-cell experiments, mRNA and protein levels for the same gene were uncorrelated. </jats:p>
収録刊行物
-
- Science
-
Science 329 (5991), 533-538, 2010-07-30
American Association for the Advancement of Science (AAAS)
