Putative involvement of crustacean hyperglycemic hormone isoforms in the neuroendocrine mediation of osmoregulation in the crayfish<i>Astacus leptodactylus</i>

DOI PDF 被引用文献2件
  • Laetitia Serrano
    Laboratoire Génome, Populations, Interactions, Adaptation, UMR 5000, Equipe Adaptation Ecophysiologique et Ontogenèse,Université Montpellier II, Place E. Bataillon, CP 092, 34095 Montpellier Cédex 05, France
  • Gaëlle Blanvillain
    Laboratoire Génome, Populations, Interactions, Adaptation, UMR 5000, Equipe Adaptation Ecophysiologique et Ontogenèse,Université Montpellier II, Place E. Bataillon, CP 092, 34095 Montpellier Cédex 05, France
  • Daniel Soyez
    Groupe Biogenèse des Peptides Isomères, UMR CNRS Physiologie et Physiopathologie, Université Paris VI, CC 256, 7 Quai Saint-Bernard,75252 Paris Cédex 05, France
  • Guy Charmantier
    Laboratoire Génome, Populations, Interactions, Adaptation, UMR 5000, Equipe Adaptation Ecophysiologique et Ontogenèse,Université Montpellier II, Place E. Bataillon, CP 092, 34095 Montpellier Cédex 05, France
  • Evelyse Grousset
    Laboratoire Génome, Populations, Interactions, Adaptation, UMR 5000, Equipe Adaptation Ecophysiologique et Ontogenèse,Université Montpellier II, Place E. Bataillon, CP 092, 34095 Montpellier Cédex 05, France
  • Fabien Aujoulat
    Laboratoire Génome, Populations, Interactions, Adaptation, UMR 5000, Equipe Adaptation Ecophysiologique et Ontogenèse,Université Montpellier II, Place E. Bataillon, CP 092, 34095 Montpellier Cédex 05, France
  • Céline Spanings-Pierrot
    Laboratoire Génome, Populations, Interactions, Adaptation, UMR 5000, Equipe Adaptation Ecophysiologique et Ontogenèse,Université Montpellier II, Place E. Bataillon, CP 092, 34095 Montpellier Cédex 05, France

書誌事項

公開日
2003-03-15
DOI
  • 10.1242/jeb.00178
公開者
The Company of Biologists

この論文をさがす

説明

<jats:title>SUMMARY</jats:title><jats:p>This study investigates the involvement of eyestalk neuroendocrine factors on osmoregulation in the crayfish Astacus leptodactylus maintained in freshwater. Eyestalk removal was followed by a significant decrease in hemolymph osmolality and Na+ concentration and by a 50% increase in mass after one molting cycle. Several neurohormones have been isolated from the sinus gland through high-performance liquid chromatography (HPLC), and different crustacean hyperglycemic hormone (CHH)-related peptides, including stereoisomers (L-CHH and D-Phe3 CHH), have been identified by direct enzyme-linked immunosorbent assay (ELISA). A glucose quantification bioassay demonstrated a strong hyperglycemic activity following injection of the immunoreactive chromatographic fractions and showed that the D-Phe3 CHH was the most efficient. Destalked crayfish were then injected with purified CHH HPLC fractions. The D-Phe3 CHH fraction significantly increased the hemolymph osmolality and Na+ content 24 h after injection. Two other CHH-related peptides caused a smaller increase in Na+ concentration. No significant variation was observed in hemolymph Cl- concentration following injection of any of the CHH isoforms. These results constitute the first observation of the effects of a CHH isoform, specifically the D-Phe3 CHH, on osmoregulatory parameters in a freshwater crustacean. The effects of eyestalk ablation and CHH injection on osmoregulation and the identification of different CHH-related peptides and isoforms in crustaceans are discussed.</jats:p>

収録刊行物

被引用文献 (2)*注記

もっと見る

詳細情報 詳細情報について

問題の指摘

ページトップへ