Neurogenesis Is Induced by Electrical Stimulation of Human Mesenchymal Stem Cells Co‐Cultured With Mature Neuronal Cells

Sang Jun Park, Ji Sun Park, Han Na Yang, Se Won Yi, Chun‐Ho Kim, Keun‐Hong Park

doi:10.1002/mabi.201500115

Neurogenesis Is Induced by Electrical Stimulation of Human Mesenchymal Stem Cells Co‐Cultured With Mature Neuronal Cells

DOI Web Site Web Site 被引用文献1件

Sang Jun Park

Laboratory of Tissue Engineering Korea Institute of Radiological and Medical Sciences 215‐4, Gongneung Nowon Seoul‐si 139‐240 Korea
Ji Sun Park

Department of Biomedical Science, College of Life Science CHA University 6F, CHA Bio‐Complex 689 Sampyeong‐dong Bundang‐gu Seongnam‐si 463‐400 Korea
Han Na Yang

Department of Biomedical Science, College of Life Science CHA University 6F, CHA Bio‐Complex 689 Sampyeong‐dong Bundang‐gu Seongnam‐si 463‐400 Korea
Se Won Yi

Department of Biomedical Science, College of Life Science CHA University 6F, CHA Bio‐Complex 689 Sampyeong‐dong Bundang‐gu Seongnam‐si 463‐400 Korea
Chun‐Ho Kim

Laboratory of Tissue Engineering Korea Institute of Radiological and Medical Sciences 215‐4, Gongneung Nowon Seoul‐si 139‐240 Korea
Keun‐Hong Park

Department of Biomedical Science, College of Life Science CHA University 6F, CHA Bio‐Complex 689 Sampyeong‐dong Bundang‐gu Seongnam‐si 463‐400 Korea

書誌事項

公開日: 2015-07-17

権利情報

http://onlinelibrary.wiley.com/termsAndConditions#vor
http://doi.wiley.com/10.1002/tdm_license_1.1
http://onlinelibrary.wiley.com/termsAndConditions#vor

DOI

10.1002/mabi.201500115

公開者: Wiley

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説明

<jats:sec><jats:label/><jats:p>For electrical stimulation of hMSCs, gold nanoparticles were coated onto polyethyleneimine coated glass cover slips. The effects of pulsed or constant electrical stimulation upon cytotoxicity and differentiation of hMSCs were examined. The effects of co culturing hMSCs with neuronal cells were also tested. The neuronal differentiation of the stem cells was evaluated by determining the expression of neuron‐speciﬁc genes and proteins using RT‐PCR and Western blotting. Morphological changes were evaluated by scanning electron microscopy. The hMSCs co‐cultured with mature neuronal cells and stimulated with electrical shock showed the greatest level of neurite outgrowth (>150 mm) and smaller cell body sizes.<jats:inline-graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="graphic/mabi201500115-gra-0001.png" xlink:title="mabi201500115-gra-0001"/></jats:p></jats:sec>