Monitoring of the ADP/ATP Ratio by Induced Circularly Polarised Europium Luminescence

Sergey Shuvaev, Mark A. Fox, David Parker

doi:10.1002/anie.201801248

Monitoring of the ADP/ATP Ratio by Induced Circularly Polarised Europium Luminescence

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Sergey Shuvaev

Department of Chemistry Durham University South Road Durham DH1 3LE UK
Mark A. Fox

Department of Chemistry Durham University South Road Durham DH1 3LE UK
David Parker

Department of Chemistry Durham University South Road Durham DH1 3LE UK

書誌事項

公開日: 2018-05-14

権利情報

http://onlinelibrary.wiley.com/termsAndConditions#vor

DOI

10.1002/anie.201801248

公開者: Wiley

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説明

<jats:title>Abstract</jats:title><jats:p>A series of three europium complexes bearing picolyl amine moieties was found to possess differing binding affinities towards Zn<jats:sup>2+</jats:sup> and three nucleotides: AMP, ADP, and ATP. A large increase in the total emission intensity was observed upon binding Zn<jats:sup>2+</jats:sup>, followed by signal amplification upon the addition of nucleotides. The resulting adducts possessed strong induced circularly polarised emission, with ADP and ATP signals of opposite sign. Model DFT geometries of the adducts suggest the Δ diastereoisomer is preferred for ATP and the Λ isomer for ADP/AMP. This change in sign allows the ADP/ATP (or AMP/ATP) ratio to be assessed by monitoring changes in the emission dissymmetry factor, <jats:italic>g</jats:italic><jats:sub>em</jats:sub>.</jats:p>