Dual binding capabilities of anti-double-stranded DNA antibodies and anti-ribosomal phosphoprotein (P) antibodies
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- M Wolfson-Reichlin
- Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma University Health Sciences Center, Oklahoma City, Oklahoma, USA
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- M Reichlin
- Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma University Health Sciences Center, Oklahoma City, Oklahoma, USA; Rheumatology, Immunology and Allergy Section, Department of Medicine, Oklahoma University Health Sciences Center, Oklahoma City, Oklahoma, USA; 825 Northeast 13th Street, Oklahoma City, OK 73104, USA
書誌事項
- 公開日
- 2001-12
- 権利情報
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- https://journals.sagepub.com/page/policies/text-and-data-mining-license
- DOI
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- 10.1191/096120301701548508
- 公開者
- SAGE Publications
この論文をさがす
説明
<jats:p> The aimof this study is to identify distinctive properties of pathogenic anti-double stranded DNA antibodies and anti-ribosomal P antibodies. </jats:p><jats:p> The binding activity of anti-dsDNA and anti-ribosomal P antibodies to their cognate antigens in 0.15 M and 1.5 M NaCl solutions on ELISA was examined. All anti-dsDNA and anti-ribosomal P antibodies exhibited a loss of their binding activity from37.5 to 100% and from2.3 to 97.4% in high ionic strength buffers, respectively. In contrast, anti-U1RNP antibodies and anti-Ro/SSA antibodies lost from0 to 32.7% and from0 to 40.1% of their binding activity, respectively. Anti-dsDNA and anti-ribosomal P antibodies from patients with nephropathy showed significantly higher binding activity in high ionic strength buffers than those frompatients without nephropathy. Study of paired sera fromlupus nephritis patients revealed that anti-dsDNA and anti-ribosomal P antibodies frompatients during disease flare show stronger binding activity in high ionic strength buffer than those during remission. Most anti-dsDNA and anti-ribosomal P antibodies bind their antigens by ionic interactions that are sensitive to high salt. Such dual binding capability of anti-dsDNA and anti-ribosomal P antibodies may underlie their multiple cross reactivities to various epitopes and help elucidate the pathogenic potential of autoantibody subsets. </jats:p>
収録刊行物
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- Lupus
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Lupus 10 (12), 857-865, 2001-12
SAGE Publications
