Transgenic AEQUORIN Reveals Organ-Specific Cytosolic Ca2+ Responses to Anoxia in Arabidopsis thaliana Seedlings

DOI PDF 被引用文献2件
  • J. C. Sedbrook
    Laboratory of Genetics, University of Wisconsin-Madison, 445 Henry Mall, Madison, Wisconsin 53706 (J.C.S., P.H.M.)
  • P. J. Kronebusch
    Laboratory of Genetics, University of Wisconsin-Madison, 445 Henry Mall, Madison, Wisconsin 53706 (J.C.S., P.H.M.)
  • G. G. Borisy
    Laboratory of Genetics, University of Wisconsin-Madison, 445 Henry Mall, Madison, Wisconsin 53706 (J.C.S., P.H.M.)
  • A. J. Trewavas
    Laboratory of Genetics, University of Wisconsin-Madison, 445 Henry Mall, Madison, Wisconsin 53706 (J.C.S., P.H.M.)
  • P. H. Masson
    Laboratory of Genetics, University of Wisconsin-Madison, 445 Henry Mall, Madison, Wisconsin 53706 (J.C.S., P.H.M.)

書誌事項

公開日
1996-05-01
権利情報
  • https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model
DOI
  • 10.1104/pp.111.1.243
公開者
Oxford University Press (OUP)

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説明

<jats:title>Abstract</jats:title> <jats:p>Using the transgenic AEQUORIN system, we showed that the cotyledons and leaves of Arabidopsis thaliana seedlings developed a biphasic luminescence response to anoxia, indicating changes in cytosolic Ca2+ levels. A fast and transient luminescence peak occurred within minutes of anoxia, followed by a second, prolonged luminescence response that lasted 1.5 to 4 h. The Ca2+ channel blockers Gd3+, La3+, and ruthenium red (RR) partially inhibited the first response and promoted a larger and earlier second response, suggesting different origins for these responses. Both Gd3+ and RR also partially inhibited anaerobic induction of alcohol dehydrogenase gene expression. However, although anaerobic alcohol dehydrogenase gene induction occurred in seedlings exposed to water-agar medium and in roots, related luminescence responses were absent. Upon return to normoxia, the luminescence of cotyledons, leaves, and roots dropped quickly, before increasing again in a Gd3+-, La3+-, ethyleneglycol-bis(β-aminoethyl ether)-N,N′-tetraacetic acid-, and RR-sensitive fashion.</jats:p>

収録刊行物

  • Plant Physiology

    Plant Physiology 111 (1), 243-257, 1996-05-01

    Oxford University Press (OUP)

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