Determination of the Structure of a Decay Accelerating Factor-Binding Clinical Isolate of Echovirus 11 Allows Mapping of Mutants with Altered Receptor Requirements for Infection
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- Amanda D. Stuart
- Division of Virology, Department of Pathology, University of Cambridge, Cambridge CB2 1QP
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- Thomas A. McKee
- Division of Virology, Department of Pathology, University of Cambridge, Cambridge CB2 1QP
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- Pamela A. Williams
- Laboratory of Molecular Biophysics, Department of Biochemistry, University of Oxford, Oxford, OX1 3QU
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- Chris Harley
- Division of Virology, Department of Pathology, University of Cambridge, Cambridge CB2 1QP
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- Shuo Shen
- Division of Virology, Department of Pathology, University of Cambridge, Cambridge CB2 1QP
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- David I. Stuart
- Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford OX3 7BN, United Kingdom
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- T. David K. Brown
- Division of Virology, Department of Pathology, University of Cambridge, Cambridge CB2 1QP
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- Susan M. Lea
- Laboratory of Molecular Biophysics, Department of Biochemistry, University of Oxford, Oxford, OX1 3QU
書誌事項
- 公開日
- 2002-08
- 権利情報
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- https://journals.asm.org/non-commercial-tdm-license
- DOI
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- 10.1128/jvi.76.15.7694-7704.2002
- 公開者
- American Society for Microbiology
この論文をさがす
説明
<jats:title>ABSTRACT</jats:title><jats:p>We have used X-ray crystallography to determine the structure of a decay accelerating factor (DAF)-binding, clinic-derived isolate of echovirus 11 (EV11-207). The structures of the capsid proteins closely resemble those of capsid proteins of other picornaviruses. The structure allows us to interpret a series of amino acid changes produced by passaging EV11-207 in different cell lines as highlighting the locations of multiple receptor-binding sites on the virion surface. We suggest that a DAF-binding site is located at the fivefold axes of the virion, while the binding site for a distinct but as yet unidentified receptor is located within the canyon surrounding the virion fivefold axes.</jats:p>
収録刊行物
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- Journal of Virology
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Journal of Virology 76 (15), 7694-7704, 2002-08
American Society for Microbiology
