Role of acidic phosphoproteins in the partial reconstitution of the active 60 S ribosomal subunit
書誌事項
- 公開日
- 1987-05-25
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1016/0014-5793(87)80761-5
- 公開者
- Wiley
この論文をさがす
説明
<jats:p>We have recently shown that rat liver 60 S ribosomal subunits active in protein synthesis can be reconstituted from inactive core particles lacking 30% of the total proteins, mainly L10a, L12, L22, L24, A33 and the acidic phosphoproteins P1–P2, obtained by treatment of 60 S subunits with dimethylmaleic anhydride [(1987) Eur. J. Biochem. 163, 15–20]. In this study, an ethanol extract of the 60 S subunit which contains only P1–P2 was also shown to be effective in reconstitution with the DMMA‐core‐particies: it strongly stimulated the EF‐2‐dependent GTP hydrolysis and, to a lesser extent, polyphenylalanine synthesis; like the DMMA wash it shifted the thermal denaturation curve of the DMMA‐core particles towards that of control subunits. Prior dephosphorylation of the ethanol extract by alkaline phosphatase inhibited the reconstruction process.</jats:p>
収録刊行物
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- FEBS Letters
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FEBS Letters 216 (1), 83-88, 1987-05-25
Wiley
