Delimitation and properties of DNA sequence: required for the regulated expression of human interferon-β gene

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書誌事項

公開日
1985-06
権利情報
  • https://www.elsevier.com/tdm/userlicense/1.0/
  • https://www.elsevier.com/legal/tdmrep-license
DOI
  • 10.1016/s0092-8674(85)80022-2
公開者
Elsevier BV

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説明

The human interferon-beta (IFN-beta) gene contains sequences within its 5'-flanking region that mediate the virus-induced activation of mRNA transcription. A series of mutant IFN-beta genes, including the 5' deletion mutants, was constructed and introduced into mouse L929 cells. Expression study of those mutant genes demonstrates that: sequences upstream, but not downstream, of -40 from the cap site are responsible for the viral induction of the gene; that the upstream boundary of the DNA sequences required to support the maximum level of induction lies between -117 and -105 from the cap site; and that this upstream sequence shows a property similar to enhancer elements as it functions in either orientation with a latitude in distance from the cap site. Within this sequence, we note the presence of repetitious hexanucleotides (consensus: A-A-AG-TG-G-A), each of which may play a role in the maximum induction of the IFN-beta gene.

収録刊行物

  • Cell

    Cell 41 (2), 489-496, 1985-06

    Elsevier BV

被引用文献 (1)*注記

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