Macromolecular Architecture in Eukaryotic Cells Visualized by Cryoelectron Tomography

DOI Web Site 被引用文献25件

書誌事項

公開日
2002-11-08
DOI
  • 10.1126/science.1076184
公開者
American Association for the Advancement of Science (AAAS)

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説明

<jats:p> Electron tomography of vitrified cells is a noninvasive three-dimensional imaging technique that opens up new vistas for exploring the supramolecular organization of the cytoplasm. We applied this technique to <jats:italic>Dictyostelium</jats:italic> cells, focusing on the actin cytoskeleton. In actin networks reconstructed without prior removal of membranes or extraction of soluble proteins, the cross-linking of individual microfilaments, their branching angles, and membrane attachment sites can be analyzed. At a resolution of 5 to 6 nanometers, single macromolecules with distinct shapes, such as the 26 <jats:italic>S</jats:italic> proteasome, can be identified in an unperturbed cellular environment. </jats:p>

収録刊行物

  • Science

    Science 298 (5596), 1209-1213, 2002-11-08

    American Association for the Advancement of Science (AAAS)

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