Comparison of Retention Behavior between Supercritical Fluid Chromatography and Normal-Phase High-Performance Liquid Chromatography with Various Stationary Phases
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- Tsunehisa Hirose
- Nacalai Tesque, Inc., Ishibashi 617-0004 17, Kaide-cho, Muko-shi, Kyoto, Japan
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- Daniel Keck
- Nacalai Tesque, Inc., Ishibashi 617-0004 17, Kaide-cho, Muko-shi, Kyoto, Japan
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- Yoshihiro Izumi
- Division of Metabolomics, Medical Institute of Bioregulation, Kyushu University, Maidashi 812-8582 3-1-1, Higashi-ku, Fukuoka, Japan
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- Takeshi Bamba
- Division of Metabolomics, Medical Institute of Bioregulation, Kyushu University, Maidashi 812-8582 3-1-1, Higashi-ku, Fukuoka, Japan
説明
<jats:p>The retention behavior of a wide variety of stationary phases was compared in supercritical fluid chromatography (SFC) and normal-phase high-performance liquid chromatography (NP-HPLC). We also attempted to elucidate the retention behavior in SFC by investigating the selectivity of the different stationary phases. SFC separation conditions with polar stationary phases, such as silica gel (SL) and diol (Diol) phases, operate via adsorptions that include hydrophilic and ionic interactions similar to those in NP-HPLC. Moreover, non-polar stationary phases, such as pentabromophenyl (PBr), pyrenylethyl (PYE), and octadecyl (C18), could be used despite the non-polar mobile phase conditions, because the dispersion and π-π interactions were stronger in SFC than in HPLC. These results reflect the selectivity of the stationary phase and its retention factor, thus providing useful information for the selection of appropriate stationary phases for particular analytes.</jats:p>
収録刊行物
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- Molecules
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Molecules 24 (13), 2425-, 2019-07-02
MDPI AG