Cryotolerance of Bovine Blastocysts is Affected by Oocyte Maturation in Media Containing Palmitic or Stearic Acid
書誌事項
- 公開日
- 2009-01-14
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1111/j.1439-0531.2008.01084.x
- 公開者
- Wiley
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説明
<jats:title>Contents</jats:title><jats:p>In this study, non‐esterified fatty acids (NEFAs) were added during <jats:italic>in vitro</jats:italic> maturation at concentrations measured previously in follicular fluid (FF) of high‐producing dairy cows in a negative energy status to evaluate their subsequent effect on the embryos cryotolerance. Oocytes were matured for 24 h in serum‐free media with or without (negative control) the addition of NEFAs dissolved in ethanol or ethanol alone (positive control). Matured oocytes were fertilized and cultured for 7 days in synthetic oviduct fluid medium supplemented with 5% FCS. Embryos that had at least reached the blastocyst stage were vitrified by open pulled straw (OPS) vitrification. Addition of palmitic (C16 : 0) or stearic acid (C18 : 0) during oocyte maturation had significant negative effects on embryo cryotolerance, whereas ethanol or oleic acid (C18 : 1) had no effect. These <jats:italic>in vitro</jats:italic> results suggest that high NEFA concentrations in FF during a period of negative energy balance in high‐yielding dairy cows can have carry‐over effects on embryo quality.</jats:p>
収録刊行物
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- Reproduction in Domestic Animals
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Reproduction in Domestic Animals 44 (1), 140-142, 2009-01-14
Wiley
