Identification of the Target of a Transcription Activator Protein by Protein-Protein Photocrosslinking
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- Yan Chen
- Department of Chemistry and Waksman Institute, Rutgers University, New Brunswick, NJ 08855, USA.
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- Yon W. Ebright
- Department of Chemistry and Waksman Institute, Rutgers University, New Brunswick, NJ 08855, USA.
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- Richard H. Ebright
- Department of Chemistry and Waksman Institute, Rutgers University, New Brunswick, NJ 08855, USA.
説明
<jats:p> Here it is shown, with the use of protein-protein photocrosslinking, that the carboxyl-terminal region of the α subunit of RNA polymerase (RNAP) is in direct physical proximity to the activating region of the catabolite gene activator protein (CAP) in the ternary complex of the <jats:italic>lac</jats:italic> promoter, RNAP, and CAP. These results strongly support the proposal that transcription activation by CAP involves protein-protein contact between the carboxyl-terminal region of the α subunit and the activating region of CAP. </jats:p>
収録刊行物
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- Science
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Science 265 (5168), 90-92, 1994-07
American Association for the Advancement of Science (AAAS)