発がん性メチル化剤により生成したデオキシリボ核酸中の1-及び3-メチルアデニンとO^6-メチルグアニンの高速液体クロマトグラフィーによる定量(<特集>生体成分の高速液体クロマトグラフィー)

DOI NDLデジタルコレクション Web Site Web Site 被引用文献1件

書誌事項

タイトル別名
  • 発がん性メチル化剤により生成したデオキシリボ核酸中の１‐及び３‐メチルアデニンとＯ｀６´‐メチルグアニンの高速液体クロマトグラフィーによる定量
  • High performance liquid chromatographic determination of 1- and 3-methyladenines and <I>O</I><SUP>6</SUP>-methylguanine in deoxyribonucleic acid formed by carcinogenic methylating agents
  • ハツガンセイ メチルカザイ ニ ヨリ セイセイシタ デオキシリボ カクサンチュ

この論文をさがす

抄録

A high performance liquid chromatographic determi-nation of 1- and 3-methyladenines and O6-methylguanine in nucleic acid was developed. Thirty milligrams of methylated DNA was dissolved in 30 ml of water and pH of the solution was adjusted to 1.6 with 0.1 M hydrochloric acid. The solution was dialyzed against 400 ml of pH 1.6 hydrochloric acid at 37°C for 26 h. The solution was evaporated under reduced pressure to 10 ml, and 5μl of the concentrate was injected onto a high performance liquid chromatograph. The method was applied for the determination of the DNA bases produced by methylation with methylnitrosourea(MNU) or N-methyl-N'-nitro-N-nitrosoguanidine (in vitro) and of methylated bases in liver DNA of rats treated with dimethylnitrosamine or MNU (in vivo). Each methylated base was successfully detected and deter-mined in all these samples. The lower limits of de-tection of the three methylated bases were 2.5 × 10-3, 5 × 10-3, and 5 × 10-3 % of the parent major bases, adenine or guanine, respectively. The recoveries of the three methylated bases were 96.4, 96.6, and 95.8 %, respectively. These results suggest the mode of reaction of carcinogenic methylating agents on DNA.

収録刊行物

  • 分析化学

    分析化学 33 (7), 351-355, 1984

    公益社団法人 日本分析化学会

被引用文献 (1)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ