Improved Methyl Esterification for GC Fatty Acid Determination in Fats and Oils: Collaborative Group Study by the Working Group (Fatty Acid Derivative Subcommittee) Japan Oil Chemists' Society
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- HIRATA Yoshiaki
- Japan Food Research Laboratories, Tama Laboratory
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- ITO Tomonori
- Food Development Laboratory, Asahi Denka Kogyo K.K.
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- UEYAMA Koreta
- Hannan R&D Center, Fuji Oil Co., Ltd.
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- KANAMORI Hironori
- Research & Development Center, Showa Sangyo Co., Ltd.
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- KAWAMURA Kentarou
- Products Development Department, Ajinomoto Oil Mills Co., Inc.
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- HASHIMOTO Masaharu
- Research & Development Laboratory, Yoshihara Oil Mill, Ltd.
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- MATSUZAKI Hisashi
- Japan Institute of Oils and Fats Other Food Inspection, Foundation
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- TAKAHASHI Shigeyuki
- Analytical Technical Laboratory, Institute of Japan Oil Stuff Inspectors’ Corporation
Bibliographic Information
- Other Title
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- 油脂中のGC脂肪酸定量のためのメチルエステル化の改良:日本油化学会ワーキンググループによる共同グループ研究(原標題は英語)
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Abstract
An eight-Japanese laboratory collaborative study was undertaken to devise an improved safe rapid means for methyl-esterification (with potassium hydroxide-methanol as the reaction solution) for GC fatty acid determination in fats and oils. This group effort (Fatty Acid Derivative Subcommittee) was undertaken at the request of the Japan Oil Chemists’ Society. Optimal determination was initially made of parameters such as concentration and volume of the reaction solution and reaction period. By 0.2 mL 2 M potassium hydroxide-methanol as reaction solution, 98.6-102% recovery was confirmed in methyl-esterification for five kinds of triacylglycerol standards; tricaprylin, trilaurin, tripalmitin, tristearin, and triolein. Collaborative study with 8 laboratories on GC with coconut, lard, soybean, and palm oil by means of the fixed reaction conditions was carried out. Reproducibility coefficient of variation (CVR) ranged from 0.6 to 2.8% for major fatty acids with peak area ratio exceeding 10% and 1.6 to 9.6% for minor fatty acids with peak area ratio less than 10%. The present method was found to qualify tentatively as the official method of the Japan Oil Chemists’ Society.<br>
Journal
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- Journal of Oleo Science
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Journal of Oleo Science 52 (11), 589-596, 2003
Japan Oil Chemists' Society
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Details 詳細情報について
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- CRID
- 1390001204092674688
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- NII Article ID
- 10012448135
- 80016193197
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- NII Book ID
- AA11503337
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- ISSN
- 13473352
- 13458957
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- NDL BIB ID
- 6715074
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed