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Studies on the Biosynthesis of Bialaphos. Biochemical Mechanism of C-P Bond Formation: Discovery of Phosphonopyruvate Decarboxylase which Catalyzes the Formation of Phosphonoacetaldehyde from Phosphonopyruvate.
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- NAKASHITA HIDEO
- Institute of Molecular and Cellular Biosciences, The University of Tokyo Present address: The Institute of Physical and Chemical Research (RIKEN)
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- WATANABE KEI
- Institute of Molecular and Cellular Biosciences, The University of Tokyo
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- HARA OSAMU
- Pharmaceutical Research Center, Meiji Seika Kaisha Ltd.
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- HIDAKA TOMOMI
- Institute of Molecular and Cellular Biosciences, The University of Tokyo
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- SETO HARUO
- Institute of Molecular and Cellular Biosciences, The University of Tokyo
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Description
The biosynthetic step following the phosphoenolpyruvate (PEP) phosphomutase reaction which forms a C-P bond of bialaphos was proven by the identification of phosphonopyruvate (PnPy) and phosphonoacetaldehyde (PnAA) as intermediates in the culture broth of Streptomyces hygroscopicus, a producing organism of bialaphos, and by detection of enzymatic decarboxylation of PnPy to PnAA. Purified PnPy decarboxylase turned out to require thiamine diphosphate and Mg2+ as cofactors. PnPy decarboxylase drives the unfavorable forward reaction to form PnPy catalyzed by PEP phosphomutase and is suggested to be essential to C-P compound biosynthesis.
Journal
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- The Journal of Antibiotics
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The Journal of Antibiotics 50 (3), 212-219, 1997
JAPAN ANTIBIOTICS RESEARCH ASSOCIATION