書誌事項
- タイトル別名
-
- Detection of Tetracyclines by Using a Thin Layer Electrophoretic Method
- ハクソウ ヘイバン オ モチイル コウアツ デンキ エイドウホウ ニ ヨル テ
この論文をさがす
説明
An electrophoretic procedure using thin layer plates of silica-gel 60F254 (Merck) and cellulose gel (Asahi Kasei Co., Ltd., Japan) has been developed for the separation and detection of 7 tetracyclines (TC, CTC, OTC, MTC, DMCTC, DOXY and MINO). Seventeen electrolyte solutions with different pH values were examined. The results obtained were as follows.<br>1. With the silica-gel plate (10×20cm), fairly good separations of tetracyclines were obtained in 0.2M sodium phosphate (dibasic)-0.1M citric acid (20:980), pH=2.2, for 40min at 1, 000V and in 1.0M sodium carbonate-1.0M sodium bicarbonate-water (50:50:900), pH=9.8, for 60min at 500V<br>2. With the cellulose plate (10×20cm), good results were obtained when the electrophoresis was done in pyridine-acetic acid-water (1:11:89), pH=3.6, for 20min at 2, 000V and in 0.05M succinic acid-0.05M sodium tetraborate (778:222), pH=4.2, for 40min at 2, 000V.<br>3. Five tetracyclines (TC, CTC, OTC, DMCTC, MINO) except for MTC and DOXY were separated from each other under the above 4 electrophoretic conditions.<br>4. All tetracyclines on TLC plates were well detected by UV absorption measurement at 365nm.
収録刊行物
-
- 食品衛生学雑誌
-
食品衛生学雑誌 29 (3), 185-191_1, 1988
公益社団法人 日本食品衛生学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390001204223016576
-
- NII論文ID
- 40001844346
- 130003692812
-
- NII書誌ID
- AN00117741
-
- ISSN
- 18821006
- 00156426
-
- NDL書誌ID
- 3187629
-
- データソース種別
-
- JaLC
- NDLサーチ
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可