ぶどう球菌エンテロトキシン簡易検査法の応用に関する研究　Ｉ　　平板パンチ法による黄色ぶどう球菌のエンテロトキシン簡易型別法

DOI

書誌事項

タイトル別名
  • Plate punch method as a simplified detection method for staphylococcal enterotoxins.
  • ブドウ球菌エンテロトキシン簡易検出法の応用に関する研究 (第1報)

抄録

An improved method for rapid determination of enterotoxin-type of Staphylococcus aureus was developed. The procedure includes the following steps: a punched-out piece with a diameter of 8-10mm around a colony of S. aureus on the Baird-Parker medium was put in 0.5ml of phosphate buffer solution containing 0.5% bovine albumin and 2% rabbit serum. Enterotoxin (s) and coagulase extracted into the solution were detected by a reversed passive latex agglutination method using a “SET-RPLA” kit and by a micro-coagulase test, respectively.<BR>The new method showed good agreement with the usual shaking culture method: 95% in terms of enterotoxin producibility and 84% in terms of the type specificity of S. aureus. In the coagulase test, the degrees of agreement with the tube method using brain-heart infusion broth containing 5% rabbit plasma were 95% in terms of coagulase producibility and 100% in terms of coagulase typing.

収録刊行物

  • 食品衛生学雑誌

    食品衛生学雑誌 26 (1), 29-32, 1985

    公益社団法人 日本食品衛生学会

キーワード

詳細情報 詳細情報について

  • CRID
    1390001204225083904
  • NII論文ID
    130003692532
  • DOI
    10.3358/shokueishi.26.29
  • ISSN
    18821006
    00156426
  • 本文言語コード
    ja
  • データソース種別
    • JaLC
    • Crossref
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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