PCR Screening System for <i>Escherichia coli</i> O157 in Meat
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- MIYAHARA Michiko
- National Institute of Health Sciences
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- KONUMA Hirotaka
- National Institute of Health Sciences
Bibliographic Information
- Other Title
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- PCR法による食肉からの腸管出血性大腸菌O157ベロ毒素産生遺伝子の検出について
- PCRホウ ニ ヨル ショクニク カラ ノ チョウカン シュッケツセイ ダイチ
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Description
PCR was inhibited by raw meat containing blood. When raw meat was tested in broth culture at the 10% rate, the PCR system could not detect vero toxin gene of 5×107 cfu (colony forming unit)/mL of O157. PCR was also inhibited by meat extract, when the extract was mixed with the PCR sample. A DNA purification procedure which eliminates blood components is essential before PCR is conducted.
Journal
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- Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
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Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 39 (5), 315-317_1, 1998
Japanese Society for Food Hygiene and Safety
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Details 詳細情報について
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- CRID
- 1390001204226553856
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- NII Article ID
- 10009301950
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- NII Book ID
- AN00117741
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- ISSN
- 18821006
- 00156426
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- NDL BIB ID
- 4592392
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed