PCR Screening System for <i>Escherichia coli</i> O157 in Meat

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  • PCR法による食肉からの腸管出血性大腸菌O157ベロ毒素産生遺伝子の検出について
  • PCRホウ ニ ヨル ショクニク カラ ノ チョウカン シュッケツセイ ダイチ

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Abstract

PCR was inhibited by raw meat containing blood. When raw meat was tested in broth culture at the 10% rate, the PCR system could not detect vero toxin gene of 5×107 cfu (colony forming unit)/mL of O157. PCR was also inhibited by meat extract, when the extract was mixed with the PCR sample. A DNA purification procedure which eliminates blood components is essential before PCR is conducted.

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