Analysis of Residual Synthetic Antibacterials in Meat and Poultry Meat by High Performance Liquid Chromatography

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  • HORII Shozo
    The Tokyo Metropolitan Research Laboratory of Public Health
  • MIYAKAWA Hiroyuki
    The Tokyo Metropolitan Research Laboratory of Public Health
  • IGUSA Kyoko
    The Tokyo Metropolitan Research Laboratory of Public Health
  • ITOH Takeshi
    The Tokyo Metropolitan Research Laboratory of Public Health

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  • 高速液体クロマトグラフィーによる食肉中の残留合成抗菌剤の分析
  • コウソク エキタイ クロマトグラフィー ニ ヨル ショクニクチュウ ノ ザンリ

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Abstract

A simple and sensitive HPLC method is described for quantitative determination of some synthetic antibacterials in animal tissues. The compounds are divided into two groups, A and B. Tissue samples are extracted with acetonitrile and then group A is purified on a silicagel cartridge and group B by hexane partition; no other clean-up procedure is needed. Recoveries of the antibacterials spiked in animal tissue were 64-99%. The detection limits of quantitative determination by the proposed method were 0.01ppm for each antibacterial. The calibration curves for these compounds showed good linearity in the ranges of 0.5-50ng for carbadox, 1-100ng for thiamphenicol and furazolidone and 2-200ng for other drugs. Many chicken samples (total 121) which were purchased from a poultry processing plant in Tokyo were analyzed by the proposed method. Decoquinate was detected in two samples of chicken muscle at levels of 0.02 and 0.03ppm.

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