薬剤耐性菌を用いる残留抗生物質の微生物学的簡易分別同定法

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タイトル別名
  • Simplified Classification Method for Residual Antibiotics by Microbiological Assay Using Drug-Resistant Bacteria
  • ヤクザイ タイセイキン オ モチイル ザンリュウ コウセイ ブッシツ ノ ビセ

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抄録

We prepared oxytetracycline (OTC)-resistant Bacillus cereus (50μg/ml), tylosin (TS)-resistant B. subtilis (12.5μg/ml) and streptomycin (SM)-resistant B. subtilis (100μg/ml) and used the sensitive organisms for microbiological assay to detect residual antibiotics. The development of drug-resistant strains to OTC, TS and SM was carried out by the broth dilution method and the obtained drug-resistant bacteria were investigated for cross resistance to about 19 antibiotics. Each drug-resistant bacterium showed almost the same cross resistance within the same groups of antibiotics.<br>The results suggested that the application of these three drug-resistant strains and the sensitive organisms will make it possible to classify residues into tetracyclines, macrolides and aminoglycoside antibiotics, especially streptomycin.

収録刊行物

  • 食品衛生学雑誌

    食品衛生学雑誌 35 (5), 543-547_1, 1994

    公益社団法人 日本食品衛生学会

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