Effect of Media on the Detection Rate of Pressure-Injured Bacteria

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  • FUJII Tateo
    Department of Food Science and Technology, Tokyo University of Fisheries
  • SATOMI Masataka
    Department of Food Science and Technology, Tokyo University of Fisheries
  • NAKATSUKA Gaku
    Department of Food Science and Technology, Tokyo University of Fisheries
  • YAMAGUCHI Toshiki
    Department of Food Science and Technology, Tokyo University of Fisheries

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  • 加圧損傷菌の検出に及ぼす培地の影響
  • カアツ ソンショウキン ノ ケンシュツ ニ オヨボス バイチ ノ エイキョウ

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Abstract

The usefulness of various media for the detection of pressure-injured bacteria was studied. Microorganisms, such as Escherichia coli, Vibrio parahaemolyticus, and Listeria monocytogenes were pressurized to obtain a survival rate of 10-50%, and then spread on several kinds of selective and non-selective media to enumerate viable counts. Detection rates on Trypticase soy agar (TSA), Nutrient agar (NA), Anaerobic bacterial count medium (ABCM) and Bonito-peptone-glucose (BPG) medium were generally superior to those on Brain heart infusion (BHI) and Plate count agar (PCA) medium. In the case of Moraxella sp. counts on BHI were only 1/500 of those on TSA. Addition of horse blood to BHI and PCA increased the detection rates. Detection rates on selective medium were relatively low and variable depending on the selective reagents included in the medium.

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