Flow Injection Analysis of Glycerol Based on Oxygen Consumption Using Glycerol Dehydrogenase and NADH Oxidase.
-
- UKEDA Hiroyuki
- Department of Bioresources Science, Faculty of Agriculture, Kochi University
-
- FUJITA Yoshihiro
- Department of Bioresources Science, Faculty of Agriculture, Kochi University
-
- SAWAMURA Masayoshi
- Department of Bioresources Science, Faculty of Agriculture, Kochi University
-
- KUSUNOSE Hirozo
- Department of Bioresources Science, Faculty of Agriculture, Kochi University
Description
A flow injection analysis system for the determination of glycerol has been developed which incorporates a Clark-type oxygen electrode and an enzyme reactor packed with Sepharose 4B on which glycerol dehydrogenase and NADH oxidase are co-immobilized. The response of the system depended on the concentration of flavine adenine dinucleotide (FAD) added into the carrier solution [0.1M glycylglycine-KOH buffer (pH 9.2) containing 30mM ammonium sulfate]. A linear relationship between the glycerol concentration and the response current in the presence of 60μM FAD was observed over the 0.1-1.5mM range. The sampling frequency was about 25 samples/h at a flow rate of 0.5ml/min, and the relative standard deviation was 0.65% at 2.5mM glycerol level (n=10). Application of this system to commercial white and red wines gave satisfactory results compared with the F-kit method. No sample pretreatment was necessary other than dilution.
Journal
-
- Analytical Sciences
-
Analytical Sciences 10 (3), 445-448, 1994
The Japan Society for Analytical Chemistry
- Tweet
Details 詳細情報について
-
- CRID
- 1390001204254859008
-
- NII Article ID
- 130003527737
-
- COI
- 1:CAS:528:DyaK2cXkvFyrsbc%3D
-
- ISSN
- 13482246
- 09106340
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- Crossref
- CiNii Articles
-
- Abstract License Flag
- Disallowed