Flow Injection Analysis of Glycerol Based on Oxygen Consumption Using Glycerol Dehydrogenase and NADH Oxidase.
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- UKEDA Hiroyuki
- Department of Bioresources Science, Faculty of Agriculture, Kochi University
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- FUJITA Yoshihiro
- Department of Bioresources Science, Faculty of Agriculture, Kochi University
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- SAWAMURA Masayoshi
- Department of Bioresources Science, Faculty of Agriculture, Kochi University
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- KUSUNOSE Hirozo
- Department of Bioresources Science, Faculty of Agriculture, Kochi University
説明
A flow injection analysis system for the determination of glycerol has been developed which incorporates a Clark-type oxygen electrode and an enzyme reactor packed with Sepharose 4B on which glycerol dehydrogenase and NADH oxidase are co-immobilized. The response of the system depended on the concentration of flavine adenine dinucleotide (FAD) added into the carrier solution [0.1M glycylglycine-KOH buffer (pH 9.2) containing 30mM ammonium sulfate]. A linear relationship between the glycerol concentration and the response current in the presence of 60μM FAD was observed over the 0.1-1.5mM range. The sampling frequency was about 25 samples/h at a flow rate of 0.5ml/min, and the relative standard deviation was 0.65% at 2.5mM glycerol level (n=10). Application of this system to commercial white and red wines gave satisfactory results compared with the F-kit method. No sample pretreatment was necessary other than dilution.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 10 (3), 445-448, 1994
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204254859008
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- NII論文ID
- 130003527737
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- COI
- 1:CAS:528:DyaK2cXkvFyrsbc%3D
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- ISSN
- 13482246
- 09106340
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可