An Electrochemical Biosensor for Nitric Oxide Based on Silver Nanoparticles and Hemoglobin

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  • GAN Xin
    Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University
  • LIU Tao
    Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University
  • ZHU Xiaoli
    Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University
  • LI Genxi
    Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University

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タイトル別名
  • Electrochemical Biosensor for Nitric Oxide Based on Silver Nanoparticles and Hemoglobin

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抄録

A nitric oxide (NO) biosensor based on silver nanoparticles was fabricated with high sensitivity and selectivity as well as stability. Silver nanoparticles could preserve the microstructures of hemoglobin, but the electrochemical reactivity of the protein and its detection sensitivity toward NO could be greatly enhanced. Accordingly, a NO biosensor was developed. The linear concentration range was from 1.0 × 10-6 to 5.0 × 10-5 M. Its detection limit was 3.0 × 10-7 M with a sensitivity of 0.0424 µA µM-1 NO. The possible co-existing compounds would not interfere with the detection.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 20 (9), 1271-1275, 2004

    社団法人 日本分析化学会

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