Piezo Electric Sensor for Endocrine-Disrupting Chemicals Using Receptor-co-factor Interaction

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  • MURATA Masaharu
    Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
  • GOUDA Chifumi
    Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
  • YANO Kentaro
    Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
  • KUROKI Shinichiro
    Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
  • SUZUTANI Tatsuo
    Department of Microbiology, School of Medicine, Fukushima Medical University
  • KATAYAMA Yoshiki
    Department of Applied Chemistry, Graduate School of Engineering, Kyushu University

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Abstract

In vitro screening assays are useful techniques for the determination of receptor-mediated activities in environmental samples. In order to define whether environmental chemicals act as an agonist or antagonist to the human estrogen receptor (hER), we have constructed a biosensor based on ligand-inducible interactions between hER and relative proteins on a quartz crystal microbalance (QCM). The his-tagged proteins, which were expressed in E. coli by recombinant DNA technology, were immobilized on an Au-electrode with Ni(II)-mediated chemisorption using the histidine tag and thiol-modified iminodiacetic acid. The resonance-frequency change of the protein-modified electrode was caused by association or dissociation with the hER relative proteins on the surface in the presence of estrogen. These results suggest that this sensor is applicable as a large-scale screening tool for estrogenic compounds.

Journal

  • Analytical Sciences

    Analytical Sciences 19 (10), 1355-1357, 2003

    The Japan Society for Analytical Chemistry

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